Choosing Monoclonal Antibodies for Allergen Detection: How Specificity, Cross-Reactivity, and Assay Design Affect Results
An unexpected result in an allergen detection assay does not always mean that the target is absent or present at the level initially expected. The antibody, sample matrix, target accessibility, and assay format can all influence the final signal.   This is why selecting a monoclonal antibody should be considered part of assay design. The most useful antibody is not necessarily the one...
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